TABLE 3.
Distribution of most common SPATE autotransporter sequences and agn43 allelic variants among diarrheagenic E. coli strains categorized by pathotypes and phylogenetic groupa
| Pathotype and phylogenetic group | No. of strains in group | No. (%) of strains positive for presence of autotransporter sequence:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| tsh | sepA | espC | espP | eatA | agn43 | agn43K12 | agn43RS218 | agn43bCFT073 | agn43EDL933 | ||
| ETEC | |||||||||||
| A | 11 | 2 (18) | 6 (55) | 0 | 0 | 1 (9) | 10 (91) | 9 (82) | 5 (45) | 0 | 2 (18) |
| B1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 (100) | 1 (100) | 0 | 0 | 0 |
| Total | 12 | 2 (17) | 6 (50) | 0 | 0 | 1 (8) | 11 (92) | 10 (83) | 5 (42) | 0 | 2 (17) |
| EPEC | |||||||||||
| A | 1 | 0 | 0 | 1 (100) | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| B1 | 4 | 1 (25) | 1 (25) | 0 | 0 | 0 | 4 (100) | 4 (100) | 3 (75) | 0 | 1 (25) |
| B2 | 1 | 0 | 0 | 1 (100) | 0 | 0 | 1 (100) | 1 (100) | 0 | 0 | 0 |
| Total | 6 | 1 (17) | 1 (17) | 2 (33) | 0 | 0 | 5 (83) | 5 (83) | 3 (50) | 0 | 1 (17) |
| EHEC | |||||||||||
| B1 | 3 | 0 | 0 | 0 | 1 (33) | 0 | 3 (100) | 3 (100) | 1 (33) | 1 (33) | 2 (67) |
| D | 2 | 0 | 0 | 0 | 2 (100) | 0 | 2 (100) | 0 | 0 | 0 | 2 (100) |
| Total | 5 | 0 | 0 | 0 | 3 (60) | 0 | 5 (100) | 3 (60) | 1 (20) | 1 (20) | 4 (80) |
| Overall total | 23 | 3 (13) | 7 (30)b | 2 (9)c | 3 (13)c | 1 (4) | 21 (91)d | 18 (78) | 9 (39)b | 1 (4) | 7 (30)b |
a
Diarrheagenic isolates were negative for vat, sat, pic, sigA, pet, aida-1, eaa, and agn43aCFT073 sequences. The number of strains tested was too low for statistical analyses between pathotypes or phylogenetic groups among diarrheagenic strains.
b
Significantly associated with diarrheagenic E. coli compared to APEC, UPEC, or commensal strains (P < 0.005).
c
Significantly associated with diarrheagenic E. coli compared to APEC or UPEC strains (P < 0.05).
d
Significantly associated with diarrheagenic E. coli compared to APEC or commensal strains (P < 0.02).