TABLE 1.
Strains and plasmids used in this studya
| Strain or plasmid | Genotype and description | Source or reference |
|---|---|---|
| Strains | ||
| B. subtilis 168 | trpC2 | 1 |
| B. subtilis NZ8900 | trpC2 amyE::spaRK | 4 |
| B. subtilis WB800 | nprE aprE epr bpr mpr::ble nprB::bsr Δvpr wprA::hyg | 34 |
| B. subtilis NZ8900-ΔykoJ | trpC2 amyE::spaRK ykoJ::spcR | This study |
| B. subtilis HT100A | NZ8900 containing Phtra-GFP fusion integrated in the chromosome | This study |
| B. subtilis PykoJ-GFP strain | NZ8900 containing PykoJ-GFP fusion integrated in the chromosome | This study |
| L. lactis MG1363 | Plasmid-free strain | 10 |
| L. lactis NZ9000 | penN::NisRK | 16 |
| Plasmids | ||
| pSG1151 | bla cat gfp | 20 |
| pPykoJ-GFP | pSG1151::PykoJ-gfp | This study |
| pPhtrA-GFP | pSG1151::PhtrA-gfp | This study |
| pDG1726 | Vector containing spectinomycin resistance cassette | 11 |
| pXB10 | pUB110 containing β-toxin-coding region | 25 |
| pNZ8048 | Nisin vector, Emr | 9 |
| pNZbtoxoid (pNZbtox) | pNZ8048::β-toxoid | 21 |
| pNZbtoxin | pNZ8048::β-toxin | This study |
| pNZ8901 | Cloning vector containing subtilin-inducible promoter, Cmr | 4 |
| pNZ8903 | Cloning vector containing WT subtilin-inducible promoter, Emr | 4 |
| pNRS-ykoJ | pNZ8901 containing ykoJ gene | This study |
| pNRS-βtoxoid (AAA) | pNZ8903 containing β-toxoid gene | This study |
| pNRS-βtoxin (DDK) | pNZ8903 containing β-toxin gene | This study |
| pNRS-βtox-DAA | pNZ8903 containing β-tox-DAA gene | This study |
| pNRS-βtox-ADA | pNZ8903 containing β-tox-ADA gene | This study |
| pNRS-βtox-AAK | pNZ8903 containing β-tox-AAK gene | This study |
| pNRS-βtox-DDA | pNZ8903 containing β-tox-DDA gene | This study |
| pNRS-βtox-DAK | pNZ8903 containing β-tox-DAK gene | This study |
| pNRS-βtox-ADK | pNZ8903 containing β-tox-ADK gene | This study |
| pRNΔykoJ_Sp | pUC18 containing up- and downstream regions of ykoJ flanking a spectinomycin resistance cassette | This study |