FIG. 4.

Degradation of ZEN in vivo by mature T₃ seeds. (a) Dissection of maize kernels into three parts: embryos (A), endosperms (B), and pericarps (C). Photographs of dissected parts are shown in the left panel. An epifluorescent stereomicroscopic image of transgenic seeds is shown in the right panel (the bar represents 5 mm). (b) Reduced amounts of ZEN in transgenic seeds soaked in the mycotoxin solution (50 μg/ml of ZEN) at 28°C. W and T represent experiments with wild-type and transgenic seeds, respectively, which were independently carried out three times (distinguished by a number after a hyphen). The stacked bars represent the amount of ZEN (μg) detected per gram of seed in each experiment. (c) Reduced amounts of ZEN in transgenic embryos, endosperms, and pericarps soaked with the mycotoxin solution (50 μg/ml of ZEN) at 28°C. The bars represent the amount of ZEN (μg) per gram of each tissue; the error bars represent standard deviations (n = 3).