FIG. 6.

Detoxification of transgenic seeds infected with F. graminearum. The mature T₃ seeds, with wild-type (W) and transgenic (T) seeds mixed, were inoculated with the fungus and incubated for 7 to 8 days at 25°C under humid conditions (equilibrium relative humidity, 100%). After removal of the mycelia on the surface of the infected grain, the wild-type and transgenic seeds were separated and examined under the epifluorescence stereomicroscope. ZEN was extracted from the seeds and quantified by HPLC. The experiment (Exp) was carried out independently five times.