TABLE 1.
PCR primers used to generate MLST data for comparison of Enterococcus species
| Primer name | Primer sequence (5′ to 3′)b | Product length (bp) | Strain-specific GenBank accession no.a |
|---|---|---|---|
| EPW2 | ACGACTTCACCCCAATCATC (fwd) CAAAGTGACAGGTGGTGCAT (rvs) | 463 | DQ839477, DQ839478, DQ839479, DQ839480, DQ839481, DQ839482, DQ839483 |
| EPE1 | ACCGACTTCGGGTGTTACAA (fwd) GAAGCAAAATCGCGAAGAAC (rvs) | 480 | DQ839449, DQ839450, DQ839451, DQ839452, DQ839453, DQ839454, DQ839455 |
| EPH4 | GGTAGCGGAGAAATTCCAAA (fwd) CCCTTATACCGGCATTCTCA (rvs) | 490 | DQ839470, DQ839471, DQ839472, DQ839473, DQ839474, DQ839475, DQ839476 |
| EPE8 | CCCCGTACATGAAATTTGGA (fwd) GAATTTCTCCGCTACCCACA (rvs) | 456 | DQ839463, DQ839464, DQ839465, DQ839466, DQ839467, DQ839468, DQ839469 |
| EPE7 | GCGTTGGAAATTTGAGAGGA (fwd) TTCTGTGTTCGGCATGGTTA (rvs) | 421 | DQ839456, DQ839457, DQ839458, DQ839459, DQ839460, DQ839461, DQ839462 |
a
For each primer set, corresponding PCR products of seven Enterococcus species were sequenced and submitted to GenBank under the reported accession numbers.
b
fwd, forward; rvs, reverse.