TABLE 1.
Bacterial strains used for NASBA
| Bacterium | Strain and serogroup | Source,e date of collection, and type of isolate | Detection of genetic targetsf:
|
||||
|---|---|---|---|---|---|---|---|
| tcpA | ctxA | groEL | hlyA | toxR | |||
| V. cholerae O1 | O1 Inaba El Tor Cip106855 | ATTC 393 | + | + | + | + | + |
| O1 Inaba El Tor ATTC 14033 | NCTC 8457 | + | + | + | + | + | |
| VC242, O1 Ogawa El Tor | Fecesa | + | + | + | + | + | |
| VC243, O1 Ogawa El Tor | Fecesa | + | + | + | + | + | |
| VC 5/77, O1 | Peru, 1991b | + | + | + | + | + | |
| VC 2/60, O1 | Albania, 1994b | + | + | + | + | + | |
| VC 2/70, O1 | Albania, 1994b | + | + | + | + | + | |
| VC 2/67, O1 | Albania, 1994b | + | + | + | + | + | |
| VC 2/57, O1 | Albania, 1994b | + | + | + | + | + | |
| VC 2/21, O1 | Italy, 1994b | + | + | + | + | + | |
| VC 2/23, O1 | Italy, 1994b | + | + | + | + | + | |
| VC 2/26, O1 | Italy, 1994b | + | + | + | + | + | |
| VC 2/32, O1 | Italy, 1994b | + | + | + | + | + | |
| VC 6/31, O1 | Italy, 2005b,c | + | + | + | + | + | |
| VC 4/53, O1 | Italy, 1973b | + | + | + | + | + | |
| VC 4/57, O1 | Italyb; environmental, ctx positive | +/− | +/− | + | + | + | |
| VC 6/23, O1 | Italyb; environmental, ctx negative | − | − | + | + | + | |
| V. cholerae non-O1 | VC 5/47, O2 | Italyb; septicemic | +/− | +/− | + | + | + |
| VC 5/42, O158 | Italyb; septicemic | − | − | + | + | + | |
| VC 4/76, O111 | Italyb; septicemic | − | − | + | + | + | |
| VC084, non-O1 | Scampid | − | − | + | + | + | |
| VC216, non-O1 | Indiad | − | − | + | + | + | |
| VC229, non-O1 | Wounda | − | − | + | + | + | |
| VC230, non-O1 | Fecesa | − | − | + | + | + | |
| VC246, non-O1 | Norwaya; mussel | − | − | + | + | + | |
| VC344, non-O1 | Norwaya; mussel | − | − | + | + | + | |
| VC503, non-O1 | Norwayd; water | − | − | + | + | + | |
| Other Vibrio speciesd | |||||||
| V. fluvialis | VF 062 | − | − | − | − | − | |
| V. mimicus | VM 034 | − | +/− | − | − | − | |
| VM 052 | − | − | − | − | − | ||
| VM 345 | − | − | − | − | − | ||
| V. metschnikovii | VM 116 | − | − | − | − | − | |
| V. parahaemolyticus | VP 160 | − | − | − | − | − | |
| VP 363 | − | − | − | − | − | ||
| VP 438 | − | − | − | − | − | ||
| V. alginolyticus | VA 054 | − | − | − | − | − | |
| VA 647 | − | − | − | − | − | ||
| Other bacterial species | |||||||
| B. cereus | ATCC 14579 | ATCC | − | − | − | − | − |
| ATCC 10987 | ATCC | − | − | − | − | − | |
| E. coli | DSM 4230 | DSMZ | − | − | − | − | − |
| ATCC 43888, O157:H7 | CCUG 29188 | − | − | − | − | − | |
| S. enterica | ATCC 13076 Cip 82.97 | CCUG 34136 T | − | − | +/− | − | − |
| ATCC 43971 Cip 60.62 | CCUG 43971 | − | − | − | − | − | |
Strain received from J. Lassen, The Norwegian Institute of Public Health, Norway.
Strain received from A. Carotolli, Instituto Superiore di Sanita, Italy.
Imported case of V. cholerae.
Strain(s) received from L. M. Rørvik, The Norwegian School of Veterinary Science, Norway.
ATCC, American Type Culture Collection; DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen (German Collection of Microorganisms and Cell Cultures); NCTC, National Collection of Type Cultures (United Kingdom); CCUG, Culture Collection of the University of Göteborg (Sweden).
+, specific amplified product detected; −, no amplified product detected; +/−, product was amplified at a late stage of the NASBA assay with a high t value and a weak fluorescence signal.