TABLE 2.
Primers and molecular beacon probes for the V. cholerae real-time NASBA assay
| Primer or MBa | Nucleotide sequence, 5′ → 3′b | Predicted size of amplicon (bp) |
|---|---|---|
| Pvc55-1 hlyA | aattctaatacgactcactatagggcAATCTCTTCCGTCCGATCAA | 135 |
| Pvc56-2 hlyA | TGATGCTGAAGGTCAAGCAG | |
| MBvc10-hlyA | ccgatcdTCAGAAAGGCTTATGGGGTGgatcgg | |
| Pvc62-1 tcpA | aattctaatacgactcactatagggCGCTGAGACCACACCCATA | 102 |
| Pvc60-2 tcpA | GAAGAAGTTTGTAAAAGAAGAACACG | |
| MBvc11-tcpA | ccgatcAGAAAACCGGTCAAGAGGGTgatcgg | |
| Pvc64-1 ctxA | aattctaatacgactcactatagggAGAAGGTGGGTGCAGTGGCTATAACA | 151 |
| Pvc61-2 ctxA | TGATCATGCAAGAGGAACTCA | |
| MBvc-12 ctxA | ccgatcTTGTTAGGCACGATGATGGAgatcgg | |
| Pvc65-1 groEL | aattctaatacgactcactatagggATGATGTTGCCCACGCTAGA | 116 |
| Pvc66-2 groEL | GGTTATCGCTGCGGTAGAAG | |
| MBvc13-groEL | ccgatcCTGTCTGTACCTTGTGCCGAgatcgg | |
| Pvc69-1 toxR | aattctaatacgactcactatagggCGGAACCGTTTTGACGTATT | 139 |
| Pvc72-2 toxR | CTCGCAATGATTTGCATGAC | |
| MBvc14-toxR | ccgatcTTAACCCAAGCCATTTCGACgatcgg |
a
MB, molecular beacon. 5′ ends of molecular beacons were labeled with 6-carboxyfluorescein, and 3′ ends were labeled with dabsyl.
b
Nucleotide sequences of primers and molecular beacons are in capital letters; lowercase letters represent surrounding sequences.
c
T7 RNA polymerase promoter sequence.
d
Stem sequence.