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Sensitivity and reproducibility of the single-target NASBA assaysa
| Target | No. of specific NASBA products detected/no. of total reactions for concn (CFU/ml) of:
|
||||||
|---|---|---|---|---|---|---|---|
| 5 | 50 | 250 | 500 | 5 × 103 | 5 × 104 | 5 × 105 | |
| groEL | 4/6 | 6/6 | NT | 6/6 | 3/3 | 3/3 | NT |
| tcpA | 2/6 | 6/6 | NT | 6/6 | 6/6 | NT | NT |
| ctxA | No signal | 3/6 | 3/3 | 6/6 | 6/6 | 6/6 | NT |
| toxR | No signal | 5/6 | 3/3 | 6/6 | 6/6 | 6/6 | NT |
| hlyA | No signal | No signal | No signal | No signal | 1/6 | 3/6 | 6/6 |
Two separate NASBA experiments, each run in triplicate, were performed. NT, not tested.
