TABLE 4.
Detection of V. cholerae cells in spiked water samplesa
| Sample | Amplification of genetic target from V. cholerae cells at:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 5 × 103 CFU/ml
|
5 × 105 CFU/ml
|
|||||||||
| groEL | tcpA | ctxA | hlyA | toxR | groEL | tcpA | ctxA | hlyA | toxR | |
| Seawater | + | + | NT | NT | NT | + | + | NT | NT | NT |
| Freshwater lake 1 | + | + | NT | NT | NT | + | + | NT | NT | NT |
| Freshwater lake 2 | NT | NT | NT | NT | NT | + | + | + | + | + |
| PBS | + | + | NT | NT | NT | + | + | + | + | + |
a
+, specific amplified product detected; NT, not tested. Environmental water samples without spiking had no effect on amplification.