Hierarchical clustering of hot spring sediment samples with weighted and unweighted UniFrac. The percentage support for nodes supported at least 70% of the time with sequence jackknifing is indicated. The name of each sample indicates the spring (e.g., A1, A2, and A3 are different springs from the Amphitheatre Springs area, and RM is from the Roaring Mountain area), whether the sample is sulfur rich (S), iron rich (Fe), or both (FeS), and the temperature. The names and branches are colored black for S samples and gray for Fe and FeS samples. (a) Weighted UniFrac with the neighbor-joining tree and (b) unweighted UniFrac with the neighbor-joining tree.