FIG. 1.

Mortality assay of the sod mutants (A) and the complemented strains (B) of P. aeruginosa PAO1 in B. mori. Each strain was precultured in LB (a) or LPS (b). P. aeruginosa PAO1 (circles), PAO1sodM (triangles), PAO1sodB (squares), and PAO1sodMB (inverted triangles) were injected into the fourth instar of B. mori larvae at various doses (A). Similarly, the complemented strain was inoculated (B). PAO1(pBBR1MCS2), the sod mutants carrying pBBR1MCS2, the sod mutants carrying pSodM, and the sod mutant carrying pSodB are represented as circles, triangles, squares, and inverted triangles, respectively. In the inoculation test using the complemented strains, the dose was 10⁵ cells. Five larvae were injected per dilution. Each data point is an average of three replicates, and the error bar indicates the standard error of the mean.