FIG. 2.

Growth of P. aeruginosa strains in BMH broth. The strains were grown overnight in LB broth at 30°C. Three microliters of the LB culture was subcultured into 3 ml of BMH broth and incubated at 25°C until an OD₆₆₀ of 0.1 was reached. Then, a 30-μl aliquot of preculture was inoculated into 3 ml of fresh BMH broth and shaken at 70 rpm under aerobic conditions at 25°C. The OD₆₆₀ was automatically recorded with an Advantec TVS062CA biophotorecorder (Advantec Toyo Co. Ltd., Tokyo, Japan). Three experiments were repeated to ensure reproducibility and gave equivalent results. Each plot represents the average of triplicates.