Figure 2.

Functional activities of patient and control neutrophils. The release of azurophilic granules and specific granules in response to buffer only, 1 μM fMLP, or 200 ng/ml PMA in the presence of 5 μg/ml cytochalasin b was measured by the presence of either myloperoxidase (A) or lactoferrin (B) in the supernatant. The data represent the mean supernatant myloperoxidase or lactoferrin as a percentage of total cellular marker protein (±SEM) from three independent experiments. The P values were calculated by using Student's t test (*, P < 0.05). (C) The production of O₂⁻ by intact neutrophils in response to 200 ng/ml PMA, 1 μM fMLP, 1 μM PAF plus 1 μM fMLP, or 1 mg/ml opsonized zymosan (OZ) was measured as SOD-inhibitable cytochrome c reduction. The data represent the mean O₂⁻ production in nmol/min (±SEM) from 5–11 independent experiments. The P values were calculated by using Student's t test (***, P < 0.005). (D) Neutrophil polarization in response to buffer only or 25 nM fMLP. The images shown are representative of three independent experiments. Numerical values represent the mean of f-actin staining with NBD-phallacidin in MCF units ± SEM in neutrophils stimulated with 100 nM fMLP from three to six independent experiments.