TABLE 1.
Detection of indigenous pseudomonads in environmental samples by FISH and culturable counts
| Samplea | Confirmed countb | Cells detected/ml byc:
|
Pd | % of confirmed count
|
||
|---|---|---|---|---|---|---|
| Ps440MB | Ps440LP | Ps440MB | Ps440LP | |||
| AS1 | 1.2 × 104 | 1.8 × 104 (4.4 × 103) | 9.2 × 103 (6.6 × 103) | 0.118 | 151 | 76 |
| AS2 | 1.1 × 104 | 1.2 × 104 (7.1 × 103) | 4.0 × 103 (1.1 × 103) | 0.121 | 111 | 37 |
| AS3 | 3.3 × 103 | 3.9 × 103 (2.9 × 103) | 2.2 × 103 (8.5 × 102) | 0.380 | 119 | 67 |
| RW1 | 1.4 | 0.62 (0.22) | 0.36 (0.23) | 0.226 | 43 | 25 |
| RW2 | 1.7 | 1.73 (0.62) | 1.05 (0.42) | 0.193 | 103 | 62 |
a
AS, activated sludge; RW, river water.
b
Confirmed counts are 67% of the average of duplicate fluorescent CFU counts (12 out of 18 fluorescent isolates were confirmed to be pseudomonads by using restriction digest of PCR products).
c
FISH-flow cytometry counts have been converted to specific cells detected per ml of original sample. One standard deviation is shown in parentheses.
d
P values are from independent-sample t tests between cells detected by FISH with Ps440MB and Ps440LP.