TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Reference |
|---|---|---|
| Bacterial strains | ||
| NCMB 2262 | V. salmonicida type strain isolated from diseased salmon | 8 |
| EN3 | luxA insertion mutant containing pEN124 chromosomally integrated into NCMB 2262 (Camr Kanr) | This study |
| EN4 | luxA insertion mutant derived from EN3 (Kanr) | This study |
| Plasmids | ||
| pEVS79 | Derivative of pBS SK+ (Stratagene Inc.) (mob site; ColE1 ori) | 40 |
| pEVS104 | Helper plasmid with tra and trb genes (Camr) | 40 |
| pVO8 | Vector plasmid with pACYC184 ori (Camr Ermr) | 47 |
| pKV17 | Derivative of pHV200 (16); 7.9-kbp SalI fragment encoding the V. fischeri ES114 lux region (ΔluxA) | 45 |
| pEN114 | 10.8-kbp SalI fragment with a portion of the V. salmonicida lux operon ligated into pEVS79 | This study |
| pEN115 | V. salmonicida luxAB PCR product ligated into pCR2.1 (Invitrogen Inc.) | This study |
| pEN123 | V. salmonicida luxAB PCR product ligated into pEVS79 | This study |
| pEN124 | pEN123 with a transposon (Kanr) inserted 987 bp downstream of the start of the V. salmonicida luxA ORF | This study |
| pEN133 | SalI fragment with V. salmonicida lux sequence 5′ to luxC ligated into pEVS79 | This study |
| pEN134 | BamHI fragment from pEN114 (luxDABEG) ligated into pV08 | This study |
| pEN135 | V. fischeri ES114 luxCDBEG (ΔluxA) from pHV200 in frame with the lacZ promoter on pVO8 | This study |
a
Camr, chloramphenicol resistance; Ermr, erythromycin resistance; Kanr, kanamycin resistance.