TABLE 3.
Experimental conditions for studies assaying survival of enteric viruses on fomites
| Virus (reference[s]) | Suspension medium | Fomite | Temp (°C) | % Humidity | PBS or BEMa
|
FSb
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Survival (days) | T90 (days) | T99 (days) | Kic | T90 (days) | T99 (days) | Kic | |||||
| Hepatitis A virus (1, 48) | PBS or FS | Alum.d | 4 | 85-90 | >60 | 35 | >60 | 0.00278 | 45 | >60 | 0.00278 |
| Alum. | 20 | 85-90 | >60 | 35 | >60 | 0.00278 | 35 | >60 | 0.00278 | ||
| Alum. | 20 | 45-55 | >60 | 11 | 50 | 0.00278 | 15 | 60 | 0.00278 | ||
| Paper | 4 | 85-90 | >60 | <1 | 5 | 0.00278 | <1 | 7 | 0.00278 | ||
| Paper | 20 | 85-90 | >60 | <1 | 2 | 0.00278 | <1 | 6 | 0.00278 | ||
| Paper | 20 | 45-55 | >60 | <1 | 1 | 0.00278 | <1 | 4 | 0.00278 | ||
| Adenovirus 40 (1) | PBS or FS | Alum. | 4 | 85-90 | 15 | <1 | <1 | 0.011 | <1 | <1 | 0.00278 |
| Alum. | 20 | 85-90 | 15 | <1 | <1 | 0.011 | <1 | <1 | 0.00278 | ||
| Alum. | 20 | 45-55 | 15 | <1 | <1 | 0.011 | <1 | <1 | 0.083 | ||
| Paper | 4 | 85-90 | >30 | <1 | 1 | 0.00278 | <1 | 1 | 0.011 | ||
| Paper | 20 | 85-90 | >30 | <1 | 1 | 0.00278 | <1 | 1 | 0.066 | ||
| Paper | 20 | 45-55 | >30 | <1 | 1 | 0.00278 | <1 | 1 | 0.066 | ||
| Rotavirus p13 (1) | PBS or FS | Alum. | 4 | 85-90 | >60 | <1 | >60 | 0.00278 | <1 | 11 | 0.00278 |
| Alum. | 20 | 85-90 | >60 | <1 | 50 | 0.00278 | 14 | >60 | 0.00278 | ||
| Alum. | 20 | 45-55 | >60 | <1 | 11 | 0.00278 | 15 | >60 | 0.00278 | ||
| Paper | 4 | 85-90 | >60 | <1 | >50 | 0.00278 | <1 | 15 | 0.00278 | ||
| Paper | 20 | 85-90 | >60 | <1 | >60 | 0.00278 | <1 | >60 | 0.00278 | ||
| Paper | 20 | 45-55 | >60 | <1 | 2.5 | 0.00278 | <1 | 18 | 0.00278 | ||
| Astrovirus (type 4) (2) | PBS or FS | China | 4 | 85-95 | 60 | <1 | 1 | 0.0021 | 8 | 15 | 0.0021 |
| China | 20 | 7 | <1 | <1 | 0.025 | <1 | 5 | 0.025 | |||
| Paper | 4 | 90 | 5 | 15 | 0.0014 | 1 | 9 | 0.0014 | |||
| Paper | 20 | 60 | <1 | <1 | 0.0021 | <1 | <1 | 0.0138 | |||
| Feline calicivirus F9 (22) | BEM | Glass coverslip | 4 | NDe | 57 | 10 | 10 | 0.0059 | |||
| Glass | 20 | ND | 35 | <1 | 10 | 0.0119 | |||||
| Glass | 37 | ND | 7 | <1 | 1 | 0.33 | |||||
a
PBS, phosphate buffer solution (for all viruses except feline calicivirus F9); BEM, basal Eagle's medium (used only for feline calicivirus F9).
b
FS, 20% fecal suspension.
c
Inactivation coefficient (Ki) = log10 reduction in virus titer per ml = (initial viral titer − final viral titer)/survival (in hours) (64).
d
Alum., aluminum surface.
e
ND, not determined.