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SDS-PAGE of β-transaminase expressed in E. coli at different stages of purification. Proteins were separated on a 10% polyacrylamide gel in the presence of 1% SDS. Lane 1, molecular mass marker (sizes of marker proteins are designated on the left side in kDa); lane 2, cell extract of E. coli BL21(DE3); lane 3, ammonium sulfate fraction (28 to 42%); lane 4, after Q-Sepharose FF column chromatography; lane 5, after HiTrap Phenyl HP column chromatography.
