FIG. 5.

Reaction scheme for the asymmetric synthesis of enantiomerically pure β-aminocarboxylic acid and the chiral analysis of asymmetrically synthesized (3S)-amino-3-phenylpropionic acid using the coupled enzyme reaction with a lipase and the β-transaminase. The lower chromatogram was obtained with racemic 3-amino-3-phenylpropionic acid for comparison. (a) Ethylbenzoylacetate; (b) benzoylacetatic acid; (c) 3-aminobutyric acid; (d) acetoacetic acid; (e) (3R)-amino-3-phenylpropionic acid; (f) (3S)-amino-3-phenylpropionic acid.