TABLE 3.
Affiliation of sequences retrieved from bacteria isolated from live and dead cultured phyllosomas based upon a partial (>250-bp) 16S rRNA sequence
| Isolate no.a | Mediumb | Closest relative (database accession no.) | Alignment (bp) | Similarityc (%) | Taxonomic description |
|---|---|---|---|---|---|
| LCPISO1 | TCBS | Vibrio harveyi, strain S35 (AY750578.1) | 936/938 | 99 | Gammaproteobacteria |
| LCPISO2 | TCBS | Vibrio campbellii, strain 90-69B3 (AY738129.1) | 943/944 | 99 | Gammaproteobacteria |
| LCPISO3 | TCBS | Vibrio sp., strain V794 (DQ146993.1) | 937/940 | 99 | Gammaproteobacteria |
| LCPISO4 | MA | Photobacterium phosphoreum (AY292916.1) | 935/939 | 99 | Gammaproteobacteria |
| LCPISO5 | MA | Pseudoalteromonas piscicida (AF081498.1) | 944/945 | 99 | Gammaproteobacteria |
| LCPISO6 | MA | Pseudoalteromonas sp., strain S9 (U80834.1) | 906/915 | 99 | Gammaproteobacteria |
| LCPISO7 | MA | Pseudoalteromonas piscicida (AF297959.1) | 921/923 | 99 | Gammaproteobacteria |
| LCPISO8 | MA | Pseudoalteromonas sp., strain S511-1 (AB029824.1) | 826/826 | 100 | Gammaproteobacteria |
| LCPISO9 | MA | Photobacterium ganghwensis, strain FR1311 (AY960847.1) | 834/835 | 99 | Gammaproteobacteria |
| LCPISO10 | MA | Vibrio alginolyticus, strain CIP 70.65 (X74691.1) | 878/884 | 99 | Gammaproteobacteria |
| DCPISO1 | TCBS | Vibrio harveyi, strain S35 (AY750578.1) | 953/954 | 99 | Gammaproteobacteria |
| DCPISO2 | TCBS | Vibrio campbellii, strain 90-69B3 (AY738129.1) | 963/972 | 99 | Gammaproteobacteria |
| DCPISO3 | MA | Vibrio natriegens, strain 01/097 (AJ874352.1) | 951/954 | 99 | Gammaproteobacteria |
| DCPISO4 | MA | Pseudoalteromonas piscicida (AB090233.1) | 922/928 | 99 | Gammaproteobacteria |
| DCPISO5 | MA | Vibrio natriegens, strain 01/097 (AJ874352.1) | 907/908 | 99 | Gammaproteobacteria |
| DCPISO6 | MA | Photobacterium ganghwensis, strain FR1311 (AY960847.1) | 781/783 | 99 | Gammaproteobacteria |
| DCPISO7 | MA | Vibrio parahaemolyticus, strain MP-2 (AY911391.1) | 831/835 | 99 | Gammaproteobacteria |
a
LCP, live phyllosoma; DCP, dead phyllosoma.
b
MA, marine agar; TCBS, thiocitrate, bile salt, sucrose agar.
c
Sequences were aligned to the closest relative by using BLAST (3). The similarity was calculated with gaps not taken into account.