TABLE 2.

Growth characteristics of E. coli mutants with mutations in anaerobic pathways

Strain	Genotype	Specific growth rate (h−1) in mediuma:
		Aerobic		Anaerobic
		LB	Minimal	LBG	Minimal
W3110	Wild type	1.31	0.97	0.98	0.51
AH240	pflB	1.23	0.99	0.79	0.17
AH241	ldhA	1.35	0.94	0.81	0.30
AH242	pflB ldhA	1.21	0.97	NG	NG
SE2378	pflB ldhA Ana+	1.18	0.56	0.46	(0.10; 0.16)

^aLB, L broth; Minimal, glucose-minimal medium; LBG, LB plus glucose (1% [wt/vol]). The concentrations of glucose in minimal medium were 0.3% for aerobic growth and 1% (wt/vol) for anaerobic growth. The first value in parentheses represents the growth rate in glucose-minimal medium with acetate (1 mg ml⁻¹), and the second value is the growth rate in the same medium with glutamate added (1 mg ml⁻¹). NG, no growth.