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. 2007 Feb 2;73(6):1899–1907. doi: 10.1128/AEM.02391-06

FIG. 1.

FIG. 1.

MALDI mass spectra of Y. pestis analyzed with CHCA in different solvents as the matrix (matrix solvent E gave no useful signal, and the data are not shown here). Bacterial samples were treated with the solvent TFA (0.1%) combined with the solvent chloroform-methanol (1:1). A, acetonitrile-methanol-water (1:1:1) with 0.1% formic acid and 0.01 M 18-crown-6; B, acetonitrile-ethanol-water (1:1:1) with 0.1% formic acid and 0.01 M 18-crown-6; C, 2-propanol-water (1:1); D, acetonitrile-water (1:2) containing 0.1% TFA.