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MALDI-TOF mass spectra of different quantities of B. anthracis (1,000 to 10,000 Da). Bacteria were treated with 0.1% TFA combined with the solvent chloroform-methanol (1:1). The matrix solution was CHCA dissolved in acetonitrile-methanol-water (1:1:1) with 0.1% formic acid and 0.01 M 18-crown-6. The best result was obtained with 4 mg of bacterial cells.
