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Reproducibility of MALDI-TOF analysis by the protocol developed in this study. A, 12 replicate spectra obtained from different spots of the same sample of Y. pestis; B, MALDI-TOF spectra of three different analysis batches of B. anthracis. Samples were prepared under the same experimental conditions; i.e., bacteria were grown under standard conditions and then treated with solvent I and solvent II with CHCA in matrix solvent A as the matrix, and the dried-droplet method was used.
