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. 2007 Jan 10;14(3):304–311. doi: 10.1128/CVI.00363-06

FIG. 2.

FIG. 2.

In vitro protein expression following transfection with the vaccine constructs. Monolayers of HEK 293-F cell transfectants were evaluated for protein expression by in situ immunocytochemistry with a mouse anti-FLAG-alkaline phosphatase conjugate. (A) Cells transfected with the VR-1055 vector; (B) cells transfected with the IC.BVP22 construct; (C) cells transfected with the BVP22.GpIIa construct; (D) cells transfected with the IC.BVP22.GpIIa construct. Protein expression by the BVP22 and the BVP22R constructs has been reported previously (23).