TABLE 1.
Immunization protocola
| Group no. | Calf no. | DRB3 alleleb | DNA vaccinec |
|---|---|---|---|
| 1 | 3892 | 1101/1201 | IC.BVP22 |
| 3937 | 1001/1101 | IC.BVP22 | |
| 3943 | 1501/1101 | IC.BVP22 | |
| 3945 | 0101/1101 | IC.BVP22 | |
| 3959 | 1001/1101 | IC.BVP22 | |
| 2 | 3939 | 1101/1201 | BVP22R |
| 3982 | 1001/1101 | BVP22R | |
| 3985 | 1501/1101 | BVP22R | |
| 3992 | 0101/1101 | BVP22R | |
| 4000 | 1001/1101 | BVP22R | |
| 3 | 3960 | 1101/1201 | VR-1055 |
| 3962 | 1101/0201 | VR-1055 | |
| 3989 | 1101/1501 | VR-1055 | |
| 3991 | 1401/1101 | VR-1055 |
a
Calves were inoculated intradermally with the DNA molecular adjuvant constructs encoding FLT3L and GM-CSF at 2 mg each (24). The calves were then reexposed to antigen by inoculation of recombinant MSP1aBT protein at 20 μg, and recall responses were evaluated within 96 h.
b
MHC class II DRB3 alleles were defined by microarray analysis of exon 2 (27).
c
Calves were inoculated with the DNA construct encoding the antigen or the VR-1055 vector alone (each at 3 mg) at the same site where the cytokine constructs were injected.