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. Author manuscript; available in PMC: 2007 Apr 6.
Published in final edited form as: J Biol Chem. 2006 Aug 10;281(40):30299–30304. doi: 10.1074/jbc.M607007200

FIGURE 2.

FIGURE 2

Autophagy was induced under ER stress. A, UPR induction. Wild-type (SEY6210) cells were incubated in SMD with or without 3 mM DTT or 2 μg/ml TM or in SD-N as described in the legend to Fig. 1A. At the indicated times, proteins were precipitated with trichloroacetic acid and resolved by SDS-PAGE followed by immunoblotting using anti-Kar2 serum. B, GFP-Atg8 processing under ER stress. Wild-type (SEY6210) and atg1Δ (WHY1) cells expressing CEN plasmid-borne GFP-Atg8 from the endogenous ATG8 promoter were grown and processed as above, followed by immunoblotting with anti-GFP antibodies. C, precursor Ape1 maturation occurred under ER stress. The vac8Δ (YTS178) and atg1Δ (WHY1) cells were grown and processed as above, followed by immunoblotting with anti-Ape1 antiserum. WT, wild type.