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. Author manuscript; available in PMC: 2007 Mar 20.
Published in final edited form as: J Cell Sci. 2005 Jan 1;118(Pt 1):7–18. doi: 10.1242/jcs.01620

Fig. 3.

Fig. 3

Temporal order of action of cargo-packaging components. The yeast Cvt pathway is an example of specific autophagy. The resident vacuolar hydrolases Ape1 and Ams1 assemble into oligomers in the cytosol. Precursor (pr)Ape1 dodecamers further collect into a large Ape1 complex. The receptor/adaptor Atg19 binds to both the Ape1 complex (through the prApe1 propeptide) and Ams1. Atg11, a component that is not essential for autophagy, interacts with this Cvt complex and is required to connect the complex with the pre-autophagosomal structure (PAS). Atg11 also interacts with Atg1 (not shown, see Fig. 2A), but the timing of this interaction relative to cargo packaging is not known. Atg11 is not part of the final Cvt vesicle or autophagosome and presumably detaches from Atg19 at some time during or after the arrival of the Cvt complex at the PAS. Atg19 subsequently binds to Atg8-phosphatidylethanolamine, which is localized to the PAS, and this event might trigger completion of the vesicle.