Protein retrieval from the PAS. Only two proteins are known to remain associated with completed yeast Cvt vesicles or autophagosomes, the specific receptor Atg19 and Atg8-PE; other proteins that are involved in vesicle formation presumably recycle from the PAS or the autophagosome/Cvt vesicle during formation. Most Atg proteins are soluble or peripheral membrane proteins that can easily be released from the membrane surface, although the mechanism of targeting and release is not known. As shown here, retrieval of Atg9 from the PAS requires PtdIns(3)P, the PtdIns(3)P-binding protein Atg18, and Atg2, Atg13 and Atg1.