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. 2007 Mar 13;403(Pt 1):149–156. doi: 10.1042/BJ20061141

Figure 1. Effect of phosphorylation on reactivation of NDP kinase A S120G mutant.

Figure 1

(A) The S120G mutant was phosphorylated by 100 μM ATP using the regeneration system described in the text. Phosphorylation was monitored with the lactate dehydrogenase reaction. Final protein concentrations were 0.54 mg/ml (wild-type), 0.49 mg/ml (S120G) and 0.44 mg/ml (H118N). (B) Time-course for the reactivation of ATP-phosphorylated kinase. (●) ATP-phosphorylated enzyme; (○) ATP-phosphorylated enzyme dephosphorylated with 100 μM ADP; (■) phosphoramidate-phosphorylated enzyme; (□) phosphoramidate-phosphorylated enzyme, dephosphorylated with 100 μM ADP; and (▲) non-phosphorylated enzyme. Activity is expressed as the percentage of native NDP enzyme.