Figure 1. Gender- and strain-specific renal expression of Cyp4a-isoforms.

(A–D) Renal mRNA expression of Cyp4a10, Cyp4a12a, Cyp4a12b and Cyp4a14 in male and female NMRI, FVB/N, 129 Sv/J, Balb/c and C57BL/6 mice. Results shown are means±S.E.M. (n=5 in each group). Significant differences (P<0.05) between the groups are marked as follows: *, compared with other gender of the same strain; #, compared with other males; +, compared with other females; §, compared with 129 Sv/J, Balb/c and C57BL/6; ^, compared with 129 Sv/J and C57BL/6; °, compared with NMRI. (E) Specificity of the antibodies used for Western-blot analysis. Control: microsomal protein (8 μg) isolated from Sf9 cells not expressing any of the Cyp isoforms; other lanes: individual recombinant Cyp4a isoforms (0.2 pmol each); ‘ab: 4A1’: antibody raised against rat CYP4A1; ‘ab: 4a12’: antibody raised against a Cyp4a12a-specific peptide. (F) Western-blot analysis of renal Cyp4a protein expression using the primary 4A1 and 4a12 antibodies; 7.5 μg of total microsomal protein was loaded per lane. m, male; f, female.