Figure 4. Distribution of β-galactosidase (A) and cytochrome oxidase (B) after isopycnic centrifugation in a Percoll gradient, with 0.25 M sucrose as a solvent, of a total mitochondrial fraction (M+L) isolated from the liver of a saline-injected mouse (□) or of an aFas-injected mouse killed 60 min after injection (■).

The M+L fraction was layered at the top of the gradient. Centrifugation was performed at 25000 rev./min for 60 min in the Beckman rotor SW 65. Fractions are numbered from the top to the bottom of the gradient. DEVDase activities of homogenates were respectively 0 nmol/min per liver for the control and 0 and 66.1 nmol/min per liver for the aFas-injected mouse.