Figure 5. Caspase-3-like protease (DEVDase) activation (A) and unsedimentable activities of sulfite cytochrome c reductase (B), β-galactosidase (C), cathepsin C (D) and β-glucuronidase (E) at increasing time after injection of aFas (5 μg/animal).

Determinations were performed on total homogenate for DEVDase, on total homogenate and on high-speed supernatant (35000 rev./min for 40 min; Beckman rotor type 40) for sulfite cytochrome c reductase and acid hydrolases. DEVDase activity was given in nmol of methylumbelliferone released/min per liver. The unsedimentable activity was expressed as a percentage of the total activity present in the homogenate. Error bars represent the S.E.M. for at least three animals. The last values (9 h) were obtained in a single experiment.