Figure 2. Effects of MeS concentration on insulin secretion and intracellular Ca²⁺ in rat islets and islet cells.

(A) Example of MeS-stimulated insulin secretion in a column perifusion of 50–80 rat islets. (B) Ca²⁺ measurement and insulin secretion were determined in single rat islet cells prepared by dispersion of whole islets. Average levels of Ca²⁺ (○) were determined as means±S.E.M. from individual rat islet cells at each MeS concentration (n=58). Insulin secretion over 30 min (●) was measured in 48-well plates under the same conditions as used for measurement of Ca²⁺. Results are means±S.E.M. for three independent experiments.