Figure 4. Effects of MeS and glucose on NAD(P)H autofluorescence in INS-1 cells and islets.

Addition of 10 mmol/l MeS, 10 mmol/l glucose, 1 μmol/l FCCP, 1 μmol/l rotenone and 3 mmol/l NaN₃ are indicated by arrows, with fluorescence measured as described in the Materials and methods section. (A) Example of NAD(P)H responses in a population of INS-1 cells. (B) Example of the NAD(P)H image in a rat islet before (left) and after (right) MeS stimulation. Time-course of rat (C) and mouse (D) islet NAD(P)H responses to MeS and glucose. Islet data are illustrations from three independent experiments.