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. Author manuscript; available in PMC: 2008 Jan 1.
Published in final edited form as: Biochem Pharmacol. 2006 Sep 14;73(1):147–154. doi: 10.1016/j.bcp.2006.09.008

Figure 2. Effect of PMAT and SERT sense and antisense oligos on 5-HT uptake in cRNA-injected oocytes.

Figure 2

(a) Oocytes were injected with 50 nL water (open bars), 40 ng PMAT cRNA only (solid bars), 40 ng PMAT cRNA pre-hybridized with 1.5 ng sense oligos (shaded bars) or 40 ng PMAT cRNA pre-hybridized with 1.5 ng antisense oligos (gray bars). Uptake of 1 μM [3H]5-HT was performed after 1 hr incubation at 25° C. (b) Oocytes were injected with 50 nL water (open bars), 40 ng SERT cRNA only (solid bars), 40 ng SERT cRNA pre-hybridized with 1.5 ng sense oligos (shaded bars) or 40 ng SERT cRNA pre-hybridized with 1.5 ng antisense oligos (gray bars). Uptake of 20 nM [3H]5-HT was performed after 20 min incubation in Na+/Cl− buffer or mannitol buffer at 25° C Each bar represents the Mean ± S.E. (n=8–10). #, significantly different from water injected oocytes (p<0.001); *, significantly different from cRNA injected oocytes (p<0.001).