Skip to main content
. Author manuscript; available in PMC: 2008 Jan 12.
Published in final edited form as: Cell. 2007 Jan 12;128(1):129–139. doi: 10.1016/j.cell.2006.11.039

Figure 3. NEDD4-1 but not NEDD4-2 is an ubiquitin ligase for PTEN.

Figure 3

(A) NEDD4-1 but not NEDD4-2 was presented in the purified PTEN E3 fraction. Different volume of HeLa S-100 (HS100, 5 mg protein/ml) and purified PTEN E3 activity (E3) were subjected to immunoblot against NEDD4-1 and NEDD4-2 as indicated.

(B) NEDD4-1 fractions but not NEDD4-2 fractions possess PTEN E3 activity. NEDD4-1 and NEDD4-2 in HS100 were separated by Mono Q chromatography as shown by immunoblotting (top panel). The PTEN E3 activity in 5 μl of individual fractions (fractions 13 and 14 as NEDD4-1 fractions; 19 and 20 as NEDD4-2 fractions; fraction 11 as a negative control) was measured (bottom panel).