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. 2006 Nov 28;128(1):161–167. doi: 10.1016/j.mad.2006.11.021

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Fig. 4 — (A) A yeast proteome microarray containing ∼4400 GST-tagged yeast proteins printed in duplicate. The slide was probed with anti-GST antibodies followed by Cy5-labeled anti-rabbit antibodies. Forty of 48 blocks are shown. (B) A kinase assay with ³³P-γ-ATP and active kinase on a yeast proteome microarray. Dark spots represent radiolabeled phosphorylated substrates on the array. Kinases that autophosphorylate are printed in each of the 40 blocks (shown above in blue boxes) and serve as reference points on the slide.