FIG. 6.

Myriocin affects endocytic transport in trypanosomes. Bloodstream cells were cultured for 4 h (A) or 14 h (B) in the presence or absence of 200 nM myriocin. Cells were then incubated at 4°C or 37°C for 1 h in a medium containing 20 μg/ml FITC-tomato lectin, washed, and analyzed by flow cytometry. Uptake is presented as relative fluorescent intensity, in arbitrary units. Values are means ± standard deviations from three independent experiments.