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Western blot of Cdc37pWt and Cdc37pS14 mutant proteins. Cells expressing HA-tagged versions of wild-type Cdc37p or one of the three mutant forms carrying a replacement of S14 by Leu, Ala, or Glu were grown in YPD at 24°C to mid-log phase. Total-cell extracts were analyzed by Western blotting using an anti-HA antibody. After being stripped, the blots were reprobed with anti-Hsp90, stripped again, and finally probed with anti-G6PDH to assess loading.
