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. 2007 Jan 12;6(3):521–532. doi: 10.1128/EC.00343-06

FIG. 3.

FIG. 3.

Analysis of Hsp90 coimmunoprecipitation with Cdc37pS14 mutant proteins. Yeast strains expressing plasmid-encoded, HA-tagged versions of wild-type Cdc37p (DH211), Cdc37pS14A (DH212), or Cdc37S14E (DH213) were grown in YPD at 24°C to mid-log phase. Cell extracts were prepared, and an aliquot of cell-free proteins was loaded onto an anti-HA agarose column. Bound protein was eluted, and 15% of the eluate was subjected to Western blot analysis using an anti-HA or anti-Hsp90 antibody. The wild-type strain TM141, expressing untagged Cdc37p, was used as a control.