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On filters, cpnA− cells are arrested or delayed in the finger stage of development. Wild-type (WT) and cpnA− (cpnA-) cells (5 × 107) were plated on filters in starvation buffer and allowed to develop. Images were taken with a dissecting microscope at various time points after plating. (A and B) 12 h; magnification, ×7; bar = 4 mm. (C and D) 24 h; magnification, ×7; bar = 4 mm. (E and F) 48 h; magnification, ×40; bar = 0.6 mm.
