FIG. 6.

Nonglycosylated Pgp1p (Pgp1p*) is not recognized by anti-polyG antibody. (A) Western blot of Pgp1p in the PGP1-HA strain at the indicated times after tunicamycin treatment (0.1 μg/ml and 0.25 μg/ml), using anti-polyG or (after stripping of the membrane) anti-HA antibody. The control extract is from wild-type (WT) CU428 cells. Approximately the same amounts of total protein were loaded in each lane. The glycosylated (Pgp1p) and nonglycosylated (Pgp1p*) forms are indicated. (B) Expression of Pgp1p in CU428 cells treated with tunicamycin, as analyzed by Western blotting with anti-polyG or anti-HA. The control protein extract was prepared from the PGP1-HA strain treated with tunicamycin.