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. 2006 Dec 1;6(3):546–554. doi: 10.1128/EC.00330-05

FIG. 1.

FIG. 1.

Quantification of ACE1 mRNA by real-time RT-PCR during infection of barley leaves. ACE1 expression was quantified by real-time RT-PCR using RNA extracted from infected barley leaves collected at different times after droplet inoculation of Guy11 spore suspensions. ACE1 expression is calculated relative to the transcript levels of the constitutively expressed gene ILV5 using the formula Inline graphic. Each data point is the average of results from three biological replicates. Standard deviations are indicated by error bars.