FIG. 2.

ACE1 appressorium-specific expression on leaves and cellophane membranes. M. grisea Guy11 transformants carrying the promACE1::eGFP vector were used to monitor ACE1 expression as eGFP fluorescence at 16 (A), 24 (B), and 48 (C) h after inoculation of spores on barley leaves and at 24 h after inoculation of spores on a cellophane membrane (D). Bar, 10 μm. Bright field (visible): observation with a microscope at ×40 magnification under bright light. Fluorescence (blue light): observation with a microscope at ×40 magnification under UV light with an eGFP-specific filter.