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. 2006 Dec 1;6(3):546–554. doi: 10.1128/EC.00330-05

FIG. 4.

FIG. 4.

Complementation of buf1::hph mutant with BUF1 restores ACE1 expression. (A) M. grisea P1.2 buf1::hph/BUF1 transformants carrying promACE1::eGFP vector were used to monitor ACE1 expression as eGFP fluorescence. Microscopic observations were performed at ×100 magnification under bright light (A1) or UV light with an eGFP-specific filter (A2). Bar, 10 μm. (B) Pathogenicity on barley leaves of the P1.2 buf1::hph mutant (buf1::hph) and P1.2 buf1::hph mutant complemented with the BUF1 wild-type allele (buf1::hph/BUF1) and wild-type Guy11 5 days after inoculation.