TABLE 1.
ACE1 expression in appressoria differentiated on host plants or artificial membranes
| Membrane or host plant | Appressorium differentiation (%) | Appressorial turgor (MPa)a | Penetration | Appressoria expressing ACE1 (%)b | Time (h) of maximum ACE1 expression |
|---|---|---|---|---|---|
| Teflon | 95 | 4 | − | 1-5 | 24 |
| Mylar | 95 | 5.5 | − | 1-5 | 24 |
| Cellophane | 90 | ND | + | 50-75 | 72 |
| Barley | 98 | 5.5 | + | 50-75 | 24 |
| Onion | 98 | ND | + | 50-75 | 24 |
a
Turgor was determined using a cytorrhysis assay. ND, not determined.
b
eGFP fluorescence of appressoria from M. grisea Guy11 transformants carrying the promACE1::eGFP vector was monitored after inoculation of spores on barley leaves, onion epidermis, or artificial membranes.