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. 2006 Dec 1;6(3):546–554. doi: 10.1128/EC.00330-05

TABLE 2.

ACE1 expression in appressoria from M. grisea penetration-deficient mutants

M. grisea mutant Pathway altered Appressorium formation Appressorial turgora Penetration ACE1 expressionb
Guy11 Wild type + + + +
ΔcpkA cAMP signaling + + − +
Δmac1 sum1-99 cAMP signaling + ND +/− +
pls1::hph Tetraspanin + + − +
buf1::hph Melanin biosynthesis + − − −
a

Turgor was determined using cytorrhysis assays for pls1::hph (9), ΔcpkA (47), and buf1::hph (20, 47). ND, not determined.

b

eGFP fluorescence of appressoria from different M. grisea transformants carrying promACE1::eGFP vector was monitored 24 h after inoculation of spores on barley leaves.