. 2006 Dec 1;6(3):546–554. doi: 10.1128/EC.00330-05

TABLE 3.

Effect of hyperosmotic solutes on ACE1 expression in appressoria from buf1::hph melanin-deficient mutants

Molarity	Osmotic solute^a	Appressoria expressing ACE1 (%) from^b:
Molarity	Osmotic solute^a	Guy11	buf1::hph
	Water	95 ± 7	0
0.15	PEG	83 ± 2	0
1.00	Glycerol	63 ± 16	0
0.40	NaCl	84 ± 7	53 ± 14
0.60	Sucrose	81 ± 12	67 ± 7
1.00	Sorbitol	81 ± 2	58 ± 8

Spores from Guy11 wild-type transformant and P1.2 buf1::hph transformant expressing promACE1::eGFP vector were inoculated on barley leaves. After 6 h, water droplets were replaced with the osmotic solute.

Appressoria from M. grisea Guy11 or P1.2 buf1::hph transformants were monitored for eGFP fluorescence 24 h after inoculation of spores on barley leaves.