TABLE 4.
Effect of cytoskeleton polymerization inhibitors on ACE1 appressorium-specific expression
| Treatmenta | Appressorium differentiation (%) | Penetration (%)b | Appressoria expressing ACE1 (%)b | ACE1 expression inhibition rate (%) |
|---|---|---|---|---|
| Water | 100 | 57 ± 14* | 52 ± 8* | 0 |
| Carbendazim | ||||
| 30 ppm | 100 | 37 ± 7† | 41 ± 9† | 21 |
| 100 ppm | 100 | 41 ± 16† | 14 ± 7‡ | 73 |
| 300 ppm | 100 | 1 ± 1§ | 0§ | 100 |
| Cytochalasin A | ||||
| 1 μM | 100 | 54 ± 7* | 40 ± 13† | 23 |
| 3 μM | 100 | 46 ± 15† | 37 ± 13† | 29 |
| 10 μM | 84 ± 12 | 22 ± 12‡ | 17 ± 6‡ | 67 |
a
Inhibitors were applied 8 h after inoculation. eGFP fluorescence of appressoria from M. grisea Guy11 transformants carrying the promACE1::eGFP vector was monitored 24 h after inoculation of spores on onion epidermis.
b
Statistical groups indicated by symbols (*, †, ‡, and §) are based on mean comparisons using Student or Mann-Whitney tests (critical threshold α/2 = 0.025).