TABLE 1.

Differences between the EUCAST AFST and CLSI M27-A2 yeast standards

Method	Glucose supplementation (%)	Shape of well	Inoculum (CFU/ml)	Incubation time (h)	Reading	Endpoint for azole drugs
EUCAST AFST	2	Flat bottom	0.5 × 105-2.5 × 105	24	Spectrophotometric	Lowest concn of drug that inhibits growth by 50% of that of the control
CLSI M27-A2	0.2	Round bottom	0.5 × 103-2.5 × 103	48	Visual	Lowest concn of drug that inhibits growth substantially compared with that of the control